Catalog Number - 85318-05P

The 85318‑05P Cogent RP C18 ABX™ HPLC Column is engineered for robust performance under extreme mobile phase conditions, delivering consistent separations across a wide pH range (2–12).

Constructed with a bulky end‑capped C18 phase bonded to high‑purity silica, this column minimizes unwanted interactions, improving peak shape for acidic, basic, and neutral analytes, especially those with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for methods involving strong acids or bases

50 mm Length – Fast Analytical Screening & Method Development

• Designed for short run times and rapid analysis

• Ideal for method development and screening workflows

• Reduces:

  • Solvent consumption

  • Run time per sample

✅ Preferred for high‑throughput analytical environments

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • routine QC analysis

  • method development workflows

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust, reproducible analytical performance

3 µm Particle – High Efficiency & Practical Performance

• Higher efficiency than 5 µm columns

• Produces:

  • Sharper peaks

  • Improved resolution

• Lower backpressure than 1.8 µm UHPLC columns

✅ Ideal for:

• High‑performance HPLC systems

• Method development without UHPLC requirements

Wide pH Stability (pH 2–12) – Extreme Method Capabilit

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains performance under aggressive analytical conditions

✅ Provides flexibility beyond conventional C18 columns

Why Choose RP C18 ABX™ Columns (3 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and harsh environments

Balanced Efficiency & Pressure

• More efficient than 5 µm columns

• Lower pressure than 1.8 µm UHPLC columns

Improved Peak Shape for Acids & Bases

• Advanced end‑capping reduces secondary interactions

• Enhances reproducibility and accuracy

✅ Excellent for method development on standard HPLC systems

Application Performance Advantages

• Effective for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

• Enables:

  • Faster methods while maintaining resolution and performance

  • Improved separation compared to legacy 5 µm methods

✅ Ideal for optimizing existing methods and developing new workflows

System & Sample Considerations

• Compatible with standard HPLC systems

• UHPLC can be used if available, but not required

• Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain performance and lifetime

Typical Applications

• HPLC analysis

• Acidic and basic compound separations

• Pharmaceutical method development

• Stability‑indicating methods

• Long‑chain fatty acid analysis

• Routine QC and analytical workflows

Important Usage Notes

• Designed for standard HPLC analytical systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for fast method development and screening

• Monitor system pressure depending on instrument capabilities